Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth14_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth14_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 5281203 Mapping efficiency: 52.8% Sequence pairs with no alignments under any condition: 1492479 Sequence pairs did not map uniquely: 3226318 Sequence pairs which were discarded because genomic sequence could not be extracted: 28 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 1445480 ((converted) top strand) GA/CT/CT: 1198164 (complementary to (converted) top strand) GA/CT/GA: 1196249 (complementary to (converted) bottom strand) CT/GA/GA: 1441282 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 249244513 Total methylated C's in CpG context: 3369105 Total methylated C's in CHG context: 317330 Total methylated C's in CHH context: 910801 Total methylated C's in Unknown context: 8821 Total unmethylated C's in CpG context: 50401388 Total unmethylated C's in CHG context: 47125020 Total unmethylated C's in CHH context: 147120869 Total unmethylated C's in Unknown context: 255281 C methylated in CpG context: 6.3% C methylated in CHG context: 0.7% C methylated in CHH context: 0.6% C methylated in unknown context (CN or CHN): 3.3% Bismark completed in 0d 0h 41m 57s