Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth12_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth12_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 3991516 Mapping efficiency: 39.9% Sequence pairs with no alignments under any condition: 4429446 Sequence pairs did not map uniquely: 1579038 Sequence pairs which were discarded because genomic sequence could not be extracted: 42 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 933517 ((converted) top strand) GA/CT/CT: 1063256 (complementary to (converted) top strand) GA/CT/GA: 1062817 (complementary to (converted) bottom strand) CT/GA/GA: 931884 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 183553227 Total methylated C's in CpG context: 3999457 Total methylated C's in CHG context: 408487 Total methylated C's in CHH context: 1410102 Total methylated C's in Unknown context: 108277 Total unmethylated C's in CpG context: 26461797 Total unmethylated C's in CHG context: 31981147 Total unmethylated C's in CHH context: 119292237 Total unmethylated C's in Unknown context: 570311 C methylated in CpG context: 13.1% C methylated in CHG context: 1.3% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 16.0% Bismark completed in 0d 0h 35m 11s