Bismark report for: /gscratch/scrubbed/sr320/031320-TG-bs/Meth10_R1_001_val_1.fq.gz and /gscratch/scrubbed/sr320/031320-TG-bs/Meth10_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/froger/Mcap_Genome/ with the specified options: -q --score-min L,0,-0.6 -p 4 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--non_directional' specified: alignments to all strands were being performed (OT, OB, CTOT, CTOB) Final Alignment report ====================== Sequence pairs analysed in total: 10000000 Number of paired-end alignments with a unique best hit: 3970191 Mapping efficiency: 39.7% Sequence pairs with no alignments under any condition: 4247889 Sequence pairs did not map uniquely: 1781920 Sequence pairs which were discarded because genomic sequence could not be extracted: 78 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 933598 ((converted) top strand) GA/CT/CT: 1049803 (complementary to (converted) top strand) GA/CT/GA: 1053949 (complementary to (converted) bottom strand) CT/GA/GA: 932763 ((converted) bottom strand) Final Cytosine Methylation Report ================================= Total number of C's analysed: 177399257 Total methylated C's in CpG context: 3589196 Total methylated C's in CHG context: 396474 Total methylated C's in CHH context: 1417191 Total methylated C's in Unknown context: 95665 Total unmethylated C's in CpG context: 26190877 Total unmethylated C's in CHG context: 31157189 Total unmethylated C's in CHH context: 114648330 Total unmethylated C's in Unknown context: 532919 C methylated in CpG context: 12.1% C methylated in CHG context: 1.3% C methylated in CHH context: 1.2% C methylated in unknown context (CN or CHN): 15.2% Bismark completed in 0d 0h 33m 47s