Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_11_S20_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_11_S20_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11697
Pairs without alignments under any condition 22940
Pairs that did not map uniquely 5363
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 354504
Methylated C's in CpG context 34313
Methylated C's in CHG context 2018
Methylated C's in CHH context 37642
Methylated C's in Unknown context 610
Unmethylated C's in CpG context 15902
Unmethylated C's in CHG context 66016
Unmethylated C's in CHH context 198613
Unmethylated C's in Unknown context 1418
Percentage methylation (CpG context) 68.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 15.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5808 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5889 original bottom strand

Deduplication

Alignments analysed 11697
Unique alignments 9689
Duplicates removed 2008
Duplicated alignments were found at 1567 different positions

Cytosine Methylation after Extraction

Total C's analysed 156490
Methylated C's in CpG context 14276
Methylated C's in CHG context 926
Methylated C's in CHH context 19115
Unmethylated C's in CpG context 6869
Unmethylated C's in CHG context 27534
Unmethylated C's in CHH context 87770
Percentage methylation (CpG context) 67.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot