Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_08_S19_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_08_S19_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 9606
Pairs without alignments under any condition 24178
Pairs that did not map uniquely 6216
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 277885
Methylated C's in CpG context 11033
Methylated C's in CHG context 1998
Methylated C's in CHH context 68316
Methylated C's in Unknown context 794
Unmethylated C's in CpG context 21736
Unmethylated C's in CHG context 44178
Unmethylated C's in CHH context 130624
Unmethylated C's in Unknown context 1212
Percentage methylation (CpG context) 33.7%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 34.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4706 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4900 original bottom strand

Deduplication

Alignments analysed 9606
Unique alignments 8237
Duplicates removed 1369
Duplicated alignments were found at 1059 different positions

Cytosine Methylation after Extraction

Total C's analysed 130801
Methylated C's in CpG context 4800
Methylated C's in CHG context 948
Methylated C's in CHH context 36225
Unmethylated C's in CpG context 9837
Unmethylated C's in CHG context 19172
Unmethylated C's in CHH context 59819
Percentage methylation (CpG context) 32.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot