Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_01_S18_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_01_S18_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11687
Pairs without alignments under any condition 23634
Pairs that did not map uniquely 4679
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 383896
Methylated C's in CpG context 42532
Methylated C's in CHG context 2490
Methylated C's in CHH context 38281
Methylated C's in Unknown context 589
Unmethylated C's in CpG context 16123
Unmethylated C's in CHG context 74868
Unmethylated C's in CHH context 209602
Unmethylated C's in Unknown context 1273
Percentage methylation (CpG context) 72.5%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 15.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5875 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5812 original bottom strand

Deduplication

Alignments analysed 11687
Unique alignments 9830
Duplicates removed 1857
Duplicated alignments were found at 1493 different positions

Cytosine Methylation after Extraction

Total C's analysed 171157
Methylated C's in CpG context 18504
Methylated C's in CHG context 1126
Methylated C's in CHH context 18515
Unmethylated C's in CpG context 7053
Unmethylated C's in CHG context 32427
Unmethylated C's in CHH context 93532
Percentage methylation (CpG context) 72.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot