Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_28_S17_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_28_S17_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11389
Pairs without alignments under any condition 24502
Pairs that did not map uniquely 4109
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 390514
Methylated C's in CpG context 40925
Methylated C's in CHG context 2928
Methylated C's in CHH context 39564
Methylated C's in Unknown context 580
Unmethylated C's in CpG context 18433
Unmethylated C's in CHG context 76034
Unmethylated C's in CHH context 212630
Unmethylated C's in Unknown context 1250
Percentage methylation (CpG context) 68.9%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 15.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5707 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5682 original bottom strand

Deduplication

Alignments analysed 11389
Unique alignments 9280
Duplicates removed 2109
Duplicated alignments were found at 1643 different positions

Cytosine Methylation after Extraction

Total C's analysed 171591
Methylated C's in CpG context 17396
Methylated C's in CHG context 1308
Methylated C's in CHH context 18733
Unmethylated C's in CpG context 7951
Unmethylated C's in CHG context 32623
Unmethylated C's in CHH context 93580
Percentage methylation (CpG context) 68.6%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot