Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_13_S16_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_13_S16_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11972
Pairs without alignments under any condition 22519
Pairs that did not map uniquely 5509
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 440645
Methylated C's in CpG context 25717
Methylated C's in CHG context 2097
Methylated C's in CHH context 47452
Methylated C's in Unknown context 663
Unmethylated C's in CpG context 25259
Unmethylated C's in CHG context 74135
Unmethylated C's in CHH context 265985
Unmethylated C's in Unknown context 1407
Percentage methylation (CpG context) 50.4%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 15.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6003 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5969 original bottom strand

Deduplication

Alignments analysed 11972
Unique alignments 9901
Duplicates removed 2071
Duplicated alignments were found at 1570 different positions

Cytosine Methylation after Extraction

Total C's analysed 201611
Methylated C's in CpG context 11079
Methylated C's in CHG context 969
Methylated C's in CHH context 24318
Unmethylated C's in CpG context 11112
Unmethylated C's in CHG context 32341
Unmethylated C's in CHH context 121792
Percentage methylation (CpG context) 49.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot