Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_02_S15_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_02_S15_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11652
Pairs without alignments under any condition 22565
Pairs that did not map uniquely 5783
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 344906
Methylated C's in CpG context 35429
Methylated C's in CHG context 2289
Methylated C's in CHH context 46367
Methylated C's in Unknown context 802
Unmethylated C's in CpG context 15640
Unmethylated C's in CHG context 61851
Unmethylated C's in CHH context 183330
Unmethylated C's in Unknown context 1440
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 20.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5772 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5880 original bottom strand

Deduplication

Alignments analysed 11652
Unique alignments 9702
Duplicates removed 1950
Duplicated alignments were found at 1526 different positions

Cytosine Methylation after Extraction

Total C's analysed 151562
Methylated C's in CpG context 14751
Methylated C's in CHG context 1005
Methylated C's in CHH context 22570
Unmethylated C's in CpG context 6771
Unmethylated C's in CHG context 25820
Unmethylated C's in CHH context 80645
Percentage methylation (CpG context) 68.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot