Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_24_S14_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_24_S14_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8340
Pairs without alignments under any condition 25093
Pairs that did not map uniquely 6567
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 275227
Methylated C's in CpG context 2517
Methylated C's in CHG context 1156
Methylated C's in CHH context 34682
Methylated C's in Unknown context 562
Unmethylated C's in CpG context 28233
Unmethylated C's in CHG context 40274
Unmethylated C's in CHH context 168365
Unmethylated C's in Unknown context 1195
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 17.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4168 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4171 original bottom strand

Deduplication

Alignments analysed 8339
Unique alignments 7013
Duplicates removed 1326
Duplicated alignments were found at 1037 different positions

Cytosine Methylation after Extraction

Total C's analysed 133881
Methylated C's in CpG context 1114
Methylated C's in CHG context 526
Methylated C's in CHH context 18067
Unmethylated C's in CpG context 12338
Unmethylated C's in CHG context 17626
Unmethylated C's in CHH context 84210
Percentage methylation (CpG context) 8.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot