Bismark report for: /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R2_001_val_2.fq.gz (version: v0.21.0) Bismark was run with Bowtie 2 against the bisulfite genome of /gscratch/srlab/sr320/data/dumoar/sc-con-genome/ with the specified options: -q --score-min L,0,-0.6 -p 40 --reorder --ignore-quals --no-mixed --no-discordant --dovetail --maxins 500 Option '--directional' specified (default mode): alignments to complementary strands (CTOT, CTOB) were ignored (i.e. not performed) Final Alignment report ====================== Sequence pairs analysed in total: 40000 Number of paired-end alignments with a unique best hit: 7412 Mapping efficiency: 18.5% Sequence pairs with no alignments under any condition: 27584 Sequence pairs did not map uniquely: 5004 Sequence pairs which were discarded because genomic sequence could not be extracted: 0 Number of sequence pairs with unique best (first) alignment came from the bowtie output: CT/GA/CT: 3797 ((converted) top strand) GA/CT/CT: 0 (complementary to (converted) top strand) GA/CT/GA: 0 (complementary to (converted) bottom strand) CT/GA/GA: 3615 ((converted) bottom strand) Number of alignments to (merely theoretical) complementary strands being rejected in total: 0 Final Cytosine Methylation Report ================================= Total number of C's analysed: 252312 Total methylated C's in CpG context: 2793 Total methylated C's in CHG context: 1416 Total methylated C's in CHH context: 32894 Total methylated C's in Unknown context: 505 Total unmethylated C's in CpG context: 28059 Total unmethylated C's in CHG context: 41207 Total unmethylated C's in CHH context: 145943 Total unmethylated C's in Unknown context: 966 C methylated in CpG context: 9.1% C methylated in CHG context: 3.3% C methylated in CHH context: 18.4% C methylated in unknown context (CN or CHN): 34.3% Bismark completed in 0d 0h 1m 3s