Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7412
Pairs without alignments under any condition 27584
Pairs that did not map uniquely 5004
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 252312
Methylated C's in CpG context 2793
Methylated C's in CHG context 1416
Methylated C's in CHH context 32894
Methylated C's in Unknown context 505
Unmethylated C's in CpG context 28059
Unmethylated C's in CHG context 41207
Unmethylated C's in CHH context 145943
Unmethylated C's in Unknown context 966
Percentage methylation (CpG context) 9.1%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 18.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3797 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3615 original bottom strand

Deduplication

Alignments analysed 7412
Unique alignments 6031
Duplicates removed 1381
Duplicated alignments were found at 984 different positions

Cytosine Methylation after Extraction

Total C's analysed 118963
Methylated C's in CpG context 1288
Methylated C's in CHG context 647
Methylated C's in CHH context 16731
Unmethylated C's in CpG context 12459
Unmethylated C's in CHG context 18232
Unmethylated C's in CHH context 69606
Percentage methylation (CpG context) 9.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot