Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_06_S12_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_06_S12_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10961
Pairs without alignments under any condition 24453
Pairs that did not map uniquely 4586
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 384102
Methylated C's in CpG context 41302
Methylated C's in CHG context 2207
Methylated C's in CHH context 40387
Methylated C's in Unknown context 604
Unmethylated C's in CpG context 20246
Unmethylated C's in CHG context 73378
Unmethylated C's in CHH context 206582
Unmethylated C's in Unknown context 1290
Percentage methylation (CpG context) 67.1%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 16.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5491 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5470 original bottom strand

Deduplication

Alignments analysed 10961
Unique alignments 8981
Duplicates removed 1980
Duplicated alignments were found at 1512 different positions

Cytosine Methylation after Extraction

Total C's analysed 169440
Methylated C's in CpG context 17444
Methylated C's in CHG context 974
Methylated C's in CHH context 19195
Unmethylated C's in CpG context 8691
Unmethylated C's in CHG context 31410
Unmethylated C's in CHH context 91726
Percentage methylation (CpG context) 66.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot