Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_24_S11_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_24_S11_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 12165
Pairs without alignments under any condition 22303
Pairs that did not map uniquely 5532
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 377297
Methylated C's in CpG context 45207
Methylated C's in CHG context 2534
Methylated C's in CHH context 47787
Methylated C's in Unknown context 638
Unmethylated C's in CpG context 16846
Unmethylated C's in CHG context 75176
Unmethylated C's in CHH context 189747
Unmethylated C's in Unknown context 1186
Percentage methylation (CpG context) 72.9%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 20.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 6060 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 6104 original bottom strand

Deduplication

Alignments analysed 12164
Unique alignments 9991
Duplicates removed 2173
Duplicated alignments were found at 1655 different positions

Cytosine Methylation after Extraction

Total C's analysed 162790
Methylated C's in CpG context 18731
Methylated C's in CHG context 1112
Methylated C's in CHH context 23942
Unmethylated C's in CpG context 7011
Unmethylated C's in CHG context 31200
Unmethylated C's in CHH context 80794
Percentage methylation (CpG context) 72.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot