Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_21_S10_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_21_S10_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11495
Pairs without alignments under any condition 23734
Pairs that did not map uniquely 4771
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 391393
Methylated C's in CpG context 35981
Methylated C's in CHG context 2219
Methylated C's in CHH context 49721
Methylated C's in Unknown context 588
Unmethylated C's in CpG context 19350
Unmethylated C's in CHG context 74795
Unmethylated C's in CHH context 209327
Unmethylated C's in Unknown context 1278
Percentage methylation (CpG context) 65.0%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 19.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5900 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5595 original bottom strand

Deduplication

Alignments analysed 11495
Unique alignments 9209
Duplicates removed 2286
Duplicated alignments were found at 1720 different positions

Cytosine Methylation after Extraction

Total C's analysed 169414
Methylated C's in CpG context 14989
Methylated C's in CHG context 954
Methylated C's in CHH context 24580
Unmethylated C's in CpG context 8249
Unmethylated C's in CHG context 31237
Unmethylated C's in CHH context 89405
Percentage methylation (CpG context) 64.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot