Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_06_S9_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_06_S9_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7390
Pairs without alignments under any condition 27436
Pairs that did not map uniquely 5174
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 257936
Methylated C's in CpG context 2668
Methylated C's in CHG context 936
Methylated C's in CHH context 31250
Methylated C's in Unknown context 409
Unmethylated C's in CpG context 29821
Unmethylated C's in CHG context 42819
Unmethylated C's in CHH context 150442
Unmethylated C's in Unknown context 899
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 2.1%
Percentage methylation (CHH context) 17.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3690 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3700 original bottom strand

Deduplication

Alignments analysed 7390
Unique alignments 6079
Duplicates removed 1311
Duplicated alignments were found at 964 different positions

Cytosine Methylation after Extraction

Total C's analysed 124680
Methylated C's in CpG context 1234
Methylated C's in CHG context 498
Methylated C's in CHH context 17320
Unmethylated C's in CpG context 13675
Unmethylated C's in CHG context 19563
Unmethylated C's in CHH context 72390
Percentage methylation (CpG context) 8.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot