Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_01_S8_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_01_S8_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10099
Pairs without alignments under any condition 23201
Pairs that did not map uniquely 6700
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 285343
Methylated C's in CpG context 18750
Methylated C's in CHG context 1839
Methylated C's in CHH context 61919
Methylated C's in Unknown context 713
Unmethylated C's in CpG context 17118
Unmethylated C's in CHG context 48319
Unmethylated C's in CHH context 137398
Unmethylated C's in Unknown context 1139
Percentage methylation (CpG context) 52.3%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 31.1%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5077 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5022 original bottom strand

Deduplication

Alignments analysed 10099
Unique alignments 8574
Duplicates removed 1525
Duplicated alignments were found at 1205 different positions

Cytosine Methylation after Extraction

Total C's analysed 130337
Methylated C's in CpG context 8091
Methylated C's in CHG context 883
Methylated C's in CHH context 32774
Unmethylated C's in CpG context 7654
Unmethylated C's in CHG context 20771
Unmethylated C's in CHH context 60164
Percentage methylation (CpG context) 51.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot