Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_33_S7_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_33_S7_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11131
Pairs without alignments under any condition 25146
Pairs that did not map uniquely 3723
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 426598
Methylated C's in CpG context 53915
Methylated C's in CHG context 2172
Methylated C's in CHH context 29619
Methylated C's in Unknown context 433
Unmethylated C's in CpG context 18673
Unmethylated C's in CHG context 87164
Unmethylated C's in CHH context 235055
Unmethylated C's in Unknown context 1115
Percentage methylation (CpG context) 74.3%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 11.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5486 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5645 original bottom strand

Deduplication

Alignments analysed 11131
Unique alignments 9059
Duplicates removed 2072
Duplicated alignments were found at 1584 different positions

Cytosine Methylation after Extraction

Total C's analysed 187195
Methylated C's in CpG context 23336
Methylated C's in CHG context 993
Methylated C's in CHH context 14028
Unmethylated C's in CpG context 8110
Unmethylated C's in CHG context 37492
Unmethylated C's in CHH context 103236
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot