Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_15_S6_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_15_S6_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7785
Pairs without alignments under any condition 26800
Pairs that did not map uniquely 5415
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 250884
Methylated C's in CpG context 7454
Methylated C's in CHG context 1258
Methylated C's in CHH context 43702
Methylated C's in Unknown context 475
Unmethylated C's in CpG context 25801
Unmethylated C's in CHG context 40628
Unmethylated C's in CHH context 132041
Unmethylated C's in Unknown context 1014
Percentage methylation (CpG context) 22.4%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 24.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3813 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3972 original bottom strand

Deduplication

Alignments analysed 7785
Unique alignments 6387
Duplicates removed 1398
Duplicated alignments were found at 1082 different positions

Cytosine Methylation after Extraction

Total C's analysed 114939
Methylated C's in CpG context 3252
Methylated C's in CHG context 600
Methylated C's in CHH context 22662
Unmethylated C's in CpG context 11177
Unmethylated C's in CHG context 17471
Unmethylated C's in CHH context 59777
Percentage methylation (CpG context) 22.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot