Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_04_S5_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_04_S5_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8112
Pairs without alignments under any condition 24346
Pairs that did not map uniquely 7542
Genomic sequence context not extractable (edges of chromosomes) 1

Cytosine Methylation

Total C's analysed 219722
Methylated C's in CpG context 3253
Methylated C's in CHG context 1297
Methylated C's in CHH context 64705
Methylated C's in Unknown context 752
Unmethylated C's in CpG context 23088
Unmethylated C's in CHG context 29805
Unmethylated C's in CHH context 97574
Unmethylated C's in Unknown context 1081
Percentage methylation (CpG context) 12.3%
Percentage methylation (CHG context) 4.2%
Percentage methylation (CHH context) 39.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4105 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4006 original bottom strand

Deduplication

Alignments analysed 8111
Unique alignments 7039
Duplicates removed 1072
Duplicated alignments were found at 845 different positions

Cytosine Methylation after Extraction

Total C's analysed 105194
Methylated C's in CpG context 1486
Methylated C's in CHG context 666
Methylated C's in CHH context 34756
Unmethylated C's in CpG context 10447
Unmethylated C's in CHG context 13065
Unmethylated C's in CHH context 44774
Percentage methylation (CpG context) 12.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot