Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_38_S4_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_38_S4_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11180
Pairs without alignments under any condition 23867
Pairs that did not map uniquely 4953
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 343557
Methylated C's in CpG context 34977
Methylated C's in CHG context 2045
Methylated C's in CHH context 36717
Methylated C's in Unknown context 606
Unmethylated C's in CpG context 16226
Unmethylated C's in CHG context 65423
Unmethylated C's in CHH context 188169
Unmethylated C's in Unknown context 1310
Percentage methylation (CpG context) 68.3%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 16.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5592 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5588 original bottom strand

Deduplication

Alignments analysed 11180
Unique alignments 9256
Duplicates removed 1924
Duplicated alignments were found at 1513 different positions

Cytosine Methylation after Extraction

Total C's analysed 149033
Methylated C's in CpG context 14798
Methylated C's in CHG context 910
Methylated C's in CHH context 18031
Unmethylated C's in CpG context 6930
Unmethylated C's in CHG context 27509
Unmethylated C's in CHH context 80855
Percentage methylation (CpG context) 68.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot