Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_22_S3_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_22_S3_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11731
Pairs without alignments under any condition 23562
Pairs that did not map uniquely 4707
Genomic sequence context not extractable (edges of chromosomes) 2

Cytosine Methylation

Total C's analysed 412236
Methylated C's in CpG context 42748
Methylated C's in CHG context 1941
Methylated C's in CHH context 33547
Methylated C's in Unknown context 488
Unmethylated C's in CpG context 20965
Unmethylated C's in CHG context 80484
Unmethylated C's in CHH context 232551
Unmethylated C's in Unknown context 1301
Percentage methylation (CpG context) 67.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 12.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5797 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5932 original bottom strand

Deduplication

Alignments analysed 11729
Unique alignments 9711
Duplicates removed 2018
Duplicated alignments were found at 1582 different positions

Cytosine Methylation after Extraction

Total C's analysed 182739
Methylated C's in CpG context 18478
Methylated C's in CHG context 854
Methylated C's in CHH context 15981
Unmethylated C's in CpG context 8999
Unmethylated C's in CHG context 34747
Unmethylated C's in CHH context 103680
Percentage methylation (CpG context) 67.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot