Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_14_S2_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_14_S2_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10979
Pairs without alignments under any condition 23540
Pairs that did not map uniquely 5481
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 332903
Methylated C's in CpG context 34746
Methylated C's in CHG context 1632
Methylated C's in CHH context 32624
Methylated C's in Unknown context 460
Unmethylated C's in CpG context 15604
Unmethylated C's in CHG context 61648
Unmethylated C's in CHH context 186649
Unmethylated C's in Unknown context 1249
Percentage methylation (CpG context) 69.0%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 14.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5518 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5461 original bottom strand

Deduplication

Alignments analysed 10979
Unique alignments 8895
Duplicates removed 2084
Duplicated alignments were found at 1583 different positions

Cytosine Methylation after Extraction

Total C's analysed 141457
Methylated C's in CpG context 14151
Methylated C's in CHG context 742
Methylated C's in CHH context 15699
Unmethylated C's in CpG context 6550
Unmethylated C's in CHG context 25198
Unmethylated C's in CHH context 79117
Percentage methylation (CpG context) 68.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot