Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_06_S1_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_06_S1_L001_R2_001_val_2.fq.gz

Data processed at 23:17 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11352
Pairs without alignments under any condition 24337
Pairs that did not map uniquely 4311
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 409570
Methylated C's in CpG context 53224
Methylated C's in CHG context 2108
Methylated C's in CHH context 30914
Methylated C's in Unknown context 527
Unmethylated C's in CpG context 17656
Unmethylated C's in CHG context 82830
Unmethylated C's in CHH context 222838
Unmethylated C's in Unknown context 1150
Percentage methylation (CpG context) 75.1%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 12.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5605 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5747 original bottom strand

Deduplication

Alignments analysed 11352
Unique alignments 9403
Duplicates removed 1949
Duplicated alignments were found at 1480 different positions

Cytosine Methylation after Extraction

Total C's analysed 179179
Methylated C's in CpG context 22701
Methylated C's in CHG context 918
Methylated C's in CHH context 14826
Unmethylated C's in CpG context 7637
Unmethylated C's in CHG context 35353
Unmethylated C's in CHH context 97744
Percentage methylation (CpG context) 74.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot