Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_11_S20_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_11_S20_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11301
Pairs without alignments under any condition 23747
Pairs that did not map uniquely 4952
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 341524
Methylated C's in CpG context 32276
Methylated C's in CHG context 1958
Methylated C's in CHH context 37873
Methylated C's in Unknown context 589
Unmethylated C's in CpG context 15937
Unmethylated C's in CHG context 63203
Unmethylated C's in CHH context 190277
Unmethylated C's in Unknown context 1399
Percentage methylation (CpG context) 66.9%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 16.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5587 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5714 original bottom strand

Deduplication

Alignments analysed 11301
Unique alignments 9318
Duplicates removed 1983
Duplicated alignments were found at 1543 different positions

Cytosine Methylation after Extraction

Total C's analysed 149633
Methylated C's in CpG context 13408
Methylated C's in CHG context 910
Methylated C's in CHH context 18632
Unmethylated C's in CpG context 6916
Unmethylated C's in CHG context 26372
Unmethylated C's in CHH context 83395
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot