Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_08_S19_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_08_S19_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 9279
Pairs without alignments under any condition 25157
Pairs that did not map uniquely 5564
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 273135
Methylated C's in CpG context 10316
Methylated C's in CHG context 1813
Methylated C's in CHH context 67941
Methylated C's in Unknown context 825
Unmethylated C's in CpG context 21844
Unmethylated C's in CHG context 42822
Unmethylated C's in CHH context 128399
Unmethylated C's in Unknown context 1183
Percentage methylation (CpG context) 32.1%
Percentage methylation (CHG context) 4.1%
Percentage methylation (CHH context) 34.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4532 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4747 original bottom strand

Deduplication

Alignments analysed 9279
Unique alignments 7956
Duplicates removed 1323
Duplicated alignments were found at 1019 different positions

Cytosine Methylation after Extraction

Total C's analysed 128614
Methylated C's in CpG context 4466
Methylated C's in CHG context 853
Methylated C's in CHH context 36046
Unmethylated C's in CpG context 9817
Unmethylated C's in CHG context 18524
Unmethylated C's in CHH context 58908
Percentage methylation (CpG context) 31.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot