Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_01_S18_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH10_01_S18_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11202
Pairs without alignments under any condition 24587
Pairs that did not map uniquely 4211
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 367157
Methylated C's in CpG context 39840
Methylated C's in CHG context 2387
Methylated C's in CHH context 36274
Methylated C's in Unknown context 545
Unmethylated C's in CpG context 15927
Unmethylated C's in CHG context 71440
Unmethylated C's in CHH context 201289
Unmethylated C's in Unknown context 1260
Percentage methylation (CpG context) 71.4%
Percentage methylation (CHG context) 3.2%
Percentage methylation (CHH context) 15.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5634 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5568 original bottom strand

Deduplication

Alignments analysed 11202
Unique alignments 9426
Duplicates removed 1776
Duplicated alignments were found at 1428 different positions

Cytosine Methylation after Extraction

Total C's analysed 164305
Methylated C's in CpG context 17292
Methylated C's in CHG context 1073
Methylated C's in CHH context 17231
Unmethylated C's in CpG context 7026
Unmethylated C's in CHG context 30934
Unmethylated C's in CHH context 90749
Percentage methylation (CpG context) 71.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot