Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_28_S17_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_28_S17_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10849
Pairs without alignments under any condition 25410
Pairs that did not map uniquely 3741
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 370094
Methylated C's in CpG context 37967
Methylated C's in CHG context 2740
Methylated C's in CHH context 37987
Methylated C's in Unknown context 585
Unmethylated C's in CpG context 18254
Unmethylated C's in CHG context 72007
Unmethylated C's in CHH context 201139
Unmethylated C's in Unknown context 1195
Percentage methylation (CpG context) 67.5%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 15.9%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5416 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5433 original bottom strand

Deduplication

Alignments analysed 10849
Unique alignments 8828
Duplicates removed 2021
Duplicated alignments were found at 1575 different positions

Cytosine Methylation after Extraction

Total C's analysed 162498
Methylated C's in CpG context 16200
Methylated C's in CHG context 1230
Methylated C's in CHH context 18066
Unmethylated C's in CpG context 7845
Unmethylated C's in CHG context 30912
Unmethylated C's in CHH context 88245
Percentage methylation (CpG context) 67.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot