Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_13_S16_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_13_S16_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11691
Pairs without alignments under any condition 23421
Pairs that did not map uniquely 4888
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 431064
Methylated C's in CpG context 24420
Methylated C's in CHG context 2009
Methylated C's in CHH context 48147
Methylated C's in Unknown context 684
Unmethylated C's in CpG context 25606
Unmethylated C's in CHG context 71773
Unmethylated C's in CHH context 259109
Unmethylated C's in Unknown context 1396
Percentage methylation (CpG context) 48.8%
Percentage methylation (CHG context) 2.7%
Percentage methylation (CHH context) 15.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5843 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5848 original bottom strand

Deduplication

Alignments analysed 11691
Unique alignments 9638
Duplicates removed 2053
Duplicated alignments were found at 1537 different positions

Cytosine Methylation after Extraction

Total C's analysed 195511
Methylated C's in CpG context 10484
Methylated C's in CHG context 913
Methylated C's in CHH context 24109
Unmethylated C's in CpG context 11037
Unmethylated C's in CHG context 31121
Unmethylated C's in CHH context 117847
Percentage methylation (CpG context) 48.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot