Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_02_S15_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH09_02_S15_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11144
Pairs without alignments under any condition 23373
Pairs that did not map uniquely 5483
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 332075
Methylated C's in CpG context 33312
Methylated C's in CHG context 2204
Methylated C's in CHH context 47777
Methylated C's in Unknown context 769
Unmethylated C's in CpG context 15207
Unmethylated C's in CHG context 58730
Unmethylated C's in CHH context 174845
Unmethylated C's in Unknown context 1375
Percentage methylation (CpG context) 68.7%
Percentage methylation (CHG context) 3.6%
Percentage methylation (CHH context) 21.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5489 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5655 original bottom strand

Deduplication

Alignments analysed 11144
Unique alignments 9305
Duplicates removed 1839
Duplicated alignments were found at 1453 different positions

Cytosine Methylation after Extraction

Total C's analysed 146989
Methylated C's in CpG context 13923
Methylated C's in CHG context 967
Methylated C's in CHH context 23372
Unmethylated C's in CpG context 6566
Unmethylated C's in CHG context 24613
Unmethylated C's in CHH context 77548
Percentage methylation (CpG context) 68.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot