Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_24_S14_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_24_S14_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8520
Pairs without alignments under any condition 26290
Pairs that did not map uniquely 5190
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 285564
Methylated C's in CpG context 2441
Methylated C's in CHG context 1116
Methylated C's in CHH context 34424
Methylated C's in Unknown context 547
Unmethylated C's in CpG context 31542
Unmethylated C's in CHG context 42341
Unmethylated C's in CHH context 173700
Unmethylated C's in Unknown context 1324
Percentage methylation (CpG context) 7.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 16.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4281 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4239 original bottom strand

Deduplication

Alignments analysed 8520
Unique alignments 7172
Duplicates removed 1348
Duplicated alignments were found at 1059 different positions

Cytosine Methylation after Extraction

Total C's analysed 138533
Methylated C's in CpG context 1088
Methylated C's in CHG context 513
Methylated C's in CHH context 17978
Unmethylated C's in CpG context 13819
Unmethylated C's in CHG context 18500
Unmethylated C's in CHH context 86635
Percentage methylation (CpG context) 7.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot