Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_11_S13_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7454
Pairs without alignments under any condition 28610
Pairs that did not map uniquely 3936
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 257188
Methylated C's in CpG context 2676
Methylated C's in CHG context 1367
Methylated C's in CHH context 31793
Methylated C's in Unknown context 485
Unmethylated C's in CpG context 29922
Unmethylated C's in CHG context 42578
Unmethylated C's in CHH context 148852
Unmethylated C's in Unknown context 983
Percentage methylation (CpG context) 8.2%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 17.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3797 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3657 original bottom strand

Deduplication

Alignments analysed 7454
Unique alignments 6094
Duplicates removed 1360
Duplicated alignments were found at 994 different positions

Cytosine Methylation after Extraction

Total C's analysed 120655
Methylated C's in CpG context 1238
Methylated C's in CHG context 645
Methylated C's in CHH context 15719
Unmethylated C's in CpG context 13303
Unmethylated C's in CHG context 18904
Unmethylated C's in CHH context 70846
Percentage methylation (CpG context) 8.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot