Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_06_S12_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH07_06_S12_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10586
Pairs without alignments under any condition 25357
Pairs that did not map uniquely 4057
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 371313
Methylated C's in CpG context 38338
Methylated C's in CHG context 2126
Methylated C's in CHH context 39318
Methylated C's in Unknown context 603
Unmethylated C's in CpG context 20787
Unmethylated C's in CHG context 70417
Unmethylated C's in CHH context 200327
Unmethylated C's in Unknown context 1264
Percentage methylation (CpG context) 64.8%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 16.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5296 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5290 original bottom strand

Deduplication

Alignments analysed 10586
Unique alignments 8686
Duplicates removed 1900
Duplicated alignments were found at 1447 different positions

Cytosine Methylation after Extraction

Total C's analysed 164386
Methylated C's in CpG context 16239
Methylated C's in CHG context 942
Methylated C's in CHH context 18539
Unmethylated C's in CpG context 9022
Unmethylated C's in CHG context 30255
Unmethylated C's in CHH context 89389
Percentage methylation (CpG context) 64.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot