Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_24_S11_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_24_S11_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11605
Pairs without alignments under any condition 23146
Pairs that did not map uniquely 5249
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 358840
Methylated C's in CpG context 42344
Methylated C's in CHG context 2457
Methylated C's in CHH context 45257
Methylated C's in Unknown context 598
Unmethylated C's in CpG context 16609
Unmethylated C's in CHG context 71351
Unmethylated C's in CHH context 180822
Unmethylated C's in Unknown context 1200
Percentage methylation (CpG context) 71.8%
Percentage methylation (CHG context) 3.3%
Percentage methylation (CHH context) 20.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5827 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5778 original bottom strand

Deduplication

Alignments analysed 11605
Unique alignments 9546
Duplicates removed 2059
Duplicated alignments were found at 1568 different positions

Cytosine Methylation after Extraction

Total C's analysed 155000
Methylated C's in CpG context 17515
Methylated C's in CHG context 1083
Methylated C's in CHH context 22808
Unmethylated C's in CpG context 6968
Unmethylated C's in CHG context 29638
Unmethylated C's in CHH context 76988
Percentage methylation (CpG context) 71.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot