Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_21_S10_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_21_S10_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11042
Pairs without alignments under any condition 24534
Pairs that did not map uniquely 4424
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 377848
Methylated C's in CpG context 33632
Methylated C's in CHG context 2174
Methylated C's in CHH context 50208
Methylated C's in Unknown context 605
Unmethylated C's in CpG context 19353
Unmethylated C's in CHG context 71127
Unmethylated C's in CHH context 201354
Unmethylated C's in Unknown context 1326
Percentage methylation (CpG context) 63.5%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 20.0%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5664 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5378 original bottom strand

Deduplication

Alignments analysed 11042
Unique alignments 8838
Duplicates removed 2204
Duplicated alignments were found at 1655 different positions

Cytosine Methylation after Extraction

Total C's analysed 164008
Methylated C's in CpG context 14032
Methylated C's in CHG context 923
Methylated C's in CHH context 24588
Unmethylated C's in CpG context 8179
Unmethylated C's in CHG context 29710
Unmethylated C's in CHH context 86576
Percentage methylation (CpG context) 63.2%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot