Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_06_S9_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_06_S9_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7446
Pairs without alignments under any condition 28479
Pairs that did not map uniquely 4075
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 266383
Methylated C's in CpG context 2503
Methylated C's in CHG context 886
Methylated C's in CHH context 30953
Methylated C's in Unknown context 419
Unmethylated C's in CpG context 32087
Unmethylated C's in CHG context 43557
Unmethylated C's in CHH context 156397
Unmethylated C's in Unknown context 950
Percentage methylation (CpG context) 7.2%
Percentage methylation (CHG context) 2.0%
Percentage methylation (CHH context) 16.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3706 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3740 original bottom strand

Deduplication

Alignments analysed 7446
Unique alignments 6131
Duplicates removed 1315
Duplicated alignments were found at 965 different positions

Cytosine Methylation after Extraction

Total C's analysed 128426
Methylated C's in CpG context 1195
Methylated C's in CHG context 467
Methylated C's in CHH context 17120
Unmethylated C's in CpG context 14635
Unmethylated C's in CHG context 19842
Unmethylated C's in CHH context 75167
Percentage methylation (CpG context) 7.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot