Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_01_S8_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH05_01_S8_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 9690
Pairs without alignments under any condition 23982
Pairs that did not map uniquely 6328
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 274902
Methylated C's in CpG context 17640
Methylated C's in CHG context 1653
Methylated C's in CHH context 58502
Methylated C's in Unknown context 704
Unmethylated C's in CpG context 16961
Unmethylated C's in CHG context 46121
Unmethylated C's in CHH context 134025
Unmethylated C's in Unknown context 1148
Percentage methylation (CpG context) 51.0%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 30.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4866 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4824 original bottom strand

Deduplication

Alignments analysed 9690
Unique alignments 8227
Duplicates removed 1463
Duplicated alignments were found at 1159 different positions

Cytosine Methylation after Extraction

Total C's analysed 126279
Methylated C's in CpG context 7610
Methylated C's in CHG context 799
Methylated C's in CHH context 31099
Unmethylated C's in CpG context 7672
Unmethylated C's in CHG context 19815
Unmethylated C's in CHH context 59284
Percentage methylation (CpG context) 49.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot