Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_33_S7_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_33_S7_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10579
Pairs without alignments under any condition 26007
Pairs that did not map uniquely 3414
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 405282
Methylated C's in CpG context 50005
Methylated C's in CHG context 2008
Methylated C's in CHH context 28307
Methylated C's in Unknown context 457
Unmethylated C's in CpG context 18391
Unmethylated C's in CHG context 82640
Unmethylated C's in CHH context 223931
Unmethylated C's in Unknown context 1064
Percentage methylation (CpG context) 73.1%
Percentage methylation (CHG context) 2.4%
Percentage methylation (CHH context) 11.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5210 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5369 original bottom strand

Deduplication

Alignments analysed 10579
Unique alignments 8650
Duplicates removed 1929
Duplicated alignments were found at 1479 different positions

Cytosine Methylation after Extraction

Total C's analysed 178767
Methylated C's in CpG context 21761
Methylated C's in CHG context 921
Methylated C's in CHH context 13420
Unmethylated C's in CpG context 7995
Unmethylated C's in CHG context 35844
Unmethylated C's in CHH context 98826
Percentage methylation (CpG context) 73.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot