Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_15_S6_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_15_S6_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 7805
Pairs without alignments under any condition 27769
Pairs that did not map uniquely 4426
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 253302
Methylated C's in CpG context 6843
Methylated C's in CHG context 1218
Methylated C's in CHH context 39938
Methylated C's in Unknown context 441
Unmethylated C's in CpG context 27922
Unmethylated C's in CHG context 41596
Unmethylated C's in CHH context 135785
Unmethylated C's in Unknown context 1079
Percentage methylation (CpG context) 19.7%
Percentage methylation (CHG context) 2.8%
Percentage methylation (CHH context) 22.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 3810 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 3995 original bottom strand

Deduplication

Alignments analysed 7805
Unique alignments 6385
Duplicates removed 1420
Duplicated alignments were found at 1101 different positions

Cytosine Methylation after Extraction

Total C's analysed 114318
Methylated C's in CpG context 2977
Methylated C's in CHG context 559
Methylated C's in CHH context 20227
Unmethylated C's in CpG context 12097
Unmethylated C's in CHG context 17712
Unmethylated C's in CHH context 60746
Percentage methylation (CpG context) 19.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot