Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_04_S5_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH03_04_S5_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 8170
Pairs without alignments under any condition 25243
Pairs that did not map uniquely 6587
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 227949
Methylated C's in CpG context 3158
Methylated C's in CHG context 1284
Methylated C's in CHH context 67053
Methylated C's in Unknown context 808
Unmethylated C's in CpG context 24230
Unmethylated C's in CHG context 30897
Unmethylated C's in CHH context 101327
Unmethylated C's in Unknown context 1099
Percentage methylation (CpG context) 11.5%
Percentage methylation (CHG context) 4.0%
Percentage methylation (CHH context) 39.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4112 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4058 original bottom strand

Deduplication

Alignments analysed 8170
Unique alignments 7078
Duplicates removed 1092
Duplicated alignments were found at 866 different positions

Cytosine Methylation after Extraction

Total C's analysed 109311
Methylated C's in CpG context 1448
Methylated C's in CHG context 668
Methylated C's in CHH context 36515
Unmethylated C's in CpG context 10823
Unmethylated C's in CHG context 13419
Unmethylated C's in CHH context 46438
Percentage methylation (CpG context) 11.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot