Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_38_S4_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_38_S4_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10756
Pairs without alignments under any condition 24552
Pairs that did not map uniquely 4692
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 332027
Methylated C's in CpG context 32931
Methylated C's in CHG context 1933
Methylated C's in CHH context 36160
Methylated C's in Unknown context 586
Unmethylated C's in CpG context 15848
Unmethylated C's in CHG context 63167
Unmethylated C's in CHH context 181988
Unmethylated C's in Unknown context 1304
Percentage methylation (CpG context) 67.5%
Percentage methylation (CHG context) 3.0%
Percentage methylation (CHH context) 16.6%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5390 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5366 original bottom strand

Deduplication

Alignments analysed 10756
Unique alignments 8896
Duplicates removed 1860
Duplicated alignments were found at 1460 different positions

Cytosine Methylation after Extraction

Total C's analysed 144407
Methylated C's in CpG context 13962
Methylated C's in CHG context 874
Methylated C's in CHH context 17873
Unmethylated C's in CpG context 6787
Unmethylated C's in CHG context 26656
Unmethylated C's in CHH context 78255
Percentage methylation (CpG context) 67.3%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot