Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_22_S3_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_22_S3_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11096
Pairs without alignments under any condition 24527
Pairs that did not map uniquely 4377
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 388262
Methylated C's in CpG context 39461
Methylated C's in CHG context 1899
Methylated C's in CHH context 32004
Methylated C's in Unknown context 420
Unmethylated C's in CpG context 20309
Unmethylated C's in CHG context 75430
Unmethylated C's in CHH context 219159
Unmethylated C's in Unknown context 1240
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 12.7%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5467 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5629 original bottom strand

Deduplication

Alignments analysed 11096
Unique alignments 9212
Duplicates removed 1884
Duplicated alignments were found at 1474 different positions

Cytosine Methylation after Extraction

Total C's analysed 172593
Methylated C's in CpG context 17142
Methylated C's in CHG context 828
Methylated C's in CHH context 15071
Unmethylated C's in CpG context 8815
Unmethylated C's in CHG context 32711
Unmethylated C's in CHH context 98026
Percentage methylation (CpG context) 66.0%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot