Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_14_S2_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_14_S2_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10453
Pairs without alignments under any condition 24414
Pairs that did not map uniquely 5133
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 316126
Methylated C's in CpG context 32191
Methylated C's in CHG context 1533
Methylated C's in CHH context 31987
Methylated C's in Unknown context 434
Unmethylated C's in CpG context 15010
Unmethylated C's in CHG context 57875
Unmethylated C's in CHH context 177530
Unmethylated C's in Unknown context 1251
Percentage methylation (CpG context) 68.2%
Percentage methylation (CHG context) 2.6%
Percentage methylation (CHH context) 15.3%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5227 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5226 original bottom strand

Deduplication

Alignments analysed 10453
Unique alignments 8480
Duplicates removed 1973
Duplicated alignments were found at 1499 different positions

Cytosine Methylation after Extraction

Total C's analysed 135244
Methylated C's in CpG context 13149
Methylated C's in CHG context 713
Methylated C's in CHH context 15510
Unmethylated C's in CpG context 6324
Unmethylated C's in CHG context 23621
Unmethylated C's in CHH context 75927
Percentage methylation (CpG context) 67.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot