Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_06_S1_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4417/CH01_06_S1_L001_R2_001_val_2.fq.gz

Data processed at 22:47 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10749
Pairs without alignments under any condition 25236
Pairs that did not map uniquely 4015
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 386106
Methylated C's in CpG context 49564
Methylated C's in CHG context 1981
Methylated C's in CHH context 29190
Methylated C's in Unknown context 507
Unmethylated C's in CpG context 17256
Unmethylated C's in CHG context 78075
Unmethylated C's in CHH context 210040
Unmethylated C's in Unknown context 1189
Percentage methylation (CpG context) 74.2%
Percentage methylation (CHG context) 2.5%
Percentage methylation (CHH context) 12.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5277 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5472 original bottom strand

Deduplication

Alignments analysed 10749
Unique alignments 8909
Duplicates removed 1840
Duplicated alignments were found at 1388 different positions

Cytosine Methylation after Extraction

Total C's analysed 168782
Methylated C's in CpG context 21081
Methylated C's in CHG context 854
Methylated C's in CHH context 14267
Unmethylated C's in CpG context 7485
Unmethylated C's in CHG context 33316
Unmethylated C's in CHH context 91779
Percentage methylation (CpG context) 73.8%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot