Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_11_S20_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_11_S20_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11044
Pairs without alignments under any condition 23555
Pairs that did not map uniquely 5401
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 293186
Methylated C's in CpG context 27219
Methylated C's in CHG context 1716
Methylated C's in CHH context 37884
Methylated C's in Unknown context 459
Unmethylated C's in CpG context 13657
Unmethylated C's in CHG context 53292
Unmethylated C's in CHH context 159418
Unmethylated C's in Unknown context 910
Percentage methylation (CpG context) 66.6%
Percentage methylation (CHG context) 3.1%
Percentage methylation (CHH context) 19.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5474 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5570 original bottom strand

Deduplication

Alignments analysed 11044
Unique alignments 8925
Duplicates removed 2119
Duplicated alignments were found at 1605 different positions

Cytosine Methylation after Extraction

Total C's analysed 134944
Methylated C's in CpG context 12199
Methylated C's in CHG context 811
Methylated C's in CHH context 18879
Unmethylated C's in CpG context 6169
Unmethylated C's in CHG context 24026
Unmethylated C's in CHH context 72860
Percentage methylation (CpG context) 66.4%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot