Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_08_S19_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_08_S19_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 9724
Pairs without alignments under any condition 23445
Pairs that did not map uniquely 6831
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 242732
Methylated C's in CpG context 8800
Methylated C's in CHG context 1614
Methylated C's in CHH context 67924
Methylated C's in Unknown context 709
Unmethylated C's in CpG context 18574
Unmethylated C's in CHG context 35885
Unmethylated C's in CHH context 109935
Unmethylated C's in Unknown context 864
Percentage methylation (CpG context) 32.1%
Percentage methylation (CHG context) 4.3%
Percentage methylation (CHH context) 38.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 4817 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 4907 original bottom strand

Deduplication

Alignments analysed 9724
Unique alignments 8119
Duplicates removed 1605
Duplicated alignments were found at 1198 different positions

Cytosine Methylation after Extraction

Total C's analysed 124191
Methylated C's in CpG context 4261
Methylated C's in CHG context 830
Methylated C's in CHH context 37438
Unmethylated C's in CpG context 9031
Unmethylated C's in CHG context 17594
Unmethylated C's in CHH context 55037
Percentage methylation (CpG context) 32.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot