Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_01_S18_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH10_01_S18_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11362
Pairs without alignments under any condition 23986
Pairs that did not map uniquely 4652
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 317292
Methylated C's in CpG context 34001
Methylated C's in CHG context 2197
Methylated C's in CHH context 35796
Methylated C's in Unknown context 501
Unmethylated C's in CpG context 13132
Unmethylated C's in CHG context 60406
Unmethylated C's in CHH context 171760
Unmethylated C's in Unknown context 752
Percentage methylation (CpG context) 72.1%
Percentage methylation (CHG context) 3.5%
Percentage methylation (CHH context) 17.2%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5621 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5741 original bottom strand

Deduplication

Alignments analysed 11362
Unique alignments 9384
Duplicates removed 1978
Duplicated alignments were found at 1515 different positions

Cytosine Methylation after Extraction

Total C's analysed 157160
Methylated C's in CpG context 16610
Methylated C's in CHG context 1053
Methylated C's in CHH context 17717
Unmethylated C's in CpG context 6421
Unmethylated C's in CHG context 29584
Unmethylated C's in CHH context 85775
Percentage methylation (CpG context) 72.1%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot