Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_28_S17_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_28_S17_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 10269
Pairs without alignments under any condition 25783
Pairs that did not map uniquely 3948
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 290834
Methylated C's in CpG context 30663
Methylated C's in CHG context 2113
Methylated C's in CHH context 30990
Methylated C's in Unknown context 414
Unmethylated C's in CpG context 13531
Unmethylated C's in CHG context 55701
Unmethylated C's in CHH context 157836
Unmethylated C's in Unknown context 745
Percentage methylation (CpG context) 69.4%
Percentage methylation (CHG context) 3.7%
Percentage methylation (CHH context) 16.4%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5100 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5169 original bottom strand

Deduplication

Alignments analysed 10269
Unique alignments 8243
Duplicates removed 2026
Duplicated alignments were found at 1532 different positions

Cytosine Methylation after Extraction

Total C's analysed 142621
Methylated C's in CpG context 14465
Methylated C's in CHG context 1035
Methylated C's in CHH context 15301
Unmethylated C's in CpG context 6591
Unmethylated C's in CHG context 27191
Unmethylated C's in CHH context 78038
Percentage methylation (CpG context) 68.7%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot