Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_13_S16_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_13_S16_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11782
Pairs without alignments under any condition 22535
Pairs that did not map uniquely 5683
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 346147
Methylated C's in CpG context 20296
Methylated C's in CHG context 1719
Methylated C's in CHH context 46212
Methylated C's in Unknown context 447
Unmethylated C's in CpG context 20238
Unmethylated C's in CHG context 58256
Unmethylated C's in CHH context 199426
Unmethylated C's in Unknown context 811
Percentage methylation (CpG context) 50.1%
Percentage methylation (CHG context) 2.9%
Percentage methylation (CHH context) 18.8%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5960 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5822 original bottom strand

Deduplication

Alignments analysed 11782
Unique alignments 9540
Duplicates removed 2242
Duplicated alignments were found at 1643 different positions

Cytosine Methylation after Extraction

Total C's analysed 176643
Methylated C's in CpG context 9878
Methylated C's in CHG context 885
Methylated C's in CHH context 24028
Unmethylated C's in CpG context 10065
Unmethylated C's in CHG context 28745
Unmethylated C's in CHH context 103042
Percentage methylation (CpG context) 49.5%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot