Bismark

Bismark Processing Report

/gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_02_S15_L001_R1_001_val_1.fq.gz and /gscratch/srlab/lhs3/data/DuMOAR/mbdbs-trimmed/4416/CH09_02_S15_L001_R2_001_val_2.fq.gz

Data processed at 18:28 on 2023-03-12


Alignment Stats

Sequence pairs analysed in total 40000
Paired-end alignments with a unique best hit 11163
Pairs without alignments under any condition 22746
Pairs that did not map uniquely 6091
Genomic sequence context not extractable (edges of chromosomes) 0

Cytosine Methylation

Total C's analysed 290333
Methylated C's in CpG context 28519
Methylated C's in CHG context 1987
Methylated C's in CHH context 48057
Methylated C's in Unknown context 629
Unmethylated C's in CpG context 13077
Unmethylated C's in CHG context 50612
Unmethylated C's in CHH context 148081
Unmethylated C's in Unknown context 822
Percentage methylation (CpG context) 68.6%
Percentage methylation (CHG context) 3.8%
Percentage methylation (CHH context) 24.5%
Methylated C's in Unknown context N/A%

Alignment to Individual Bisulfite Strands

OT 5616 original top strand
CTOT 0 complementary to original top strand
CTOB 0 complementary to original bottom strand
OB 5547 original bottom strand

Deduplication

Alignments analysed 11163
Unique alignments 9029
Duplicates removed 2134
Duplicated alignments were found at 1573 different positions

Cytosine Methylation after Extraction

Total C's analysed 133299
Methylated C's in CpG context 12493
Methylated C's in CHG context 935
Methylated C's in CHH context 24787
Unmethylated C's in CpG context 5914
Unmethylated C's in CHG context 22095
Unmethylated C's in CHH context 67075
Percentage methylation (CpG context) 67.9%
Percentage methylation (CHG context) N/A%
Percentage methylation (CHH context) N/A%

M-Bias Plot